Htr3a^tm1Ckn
Targeted Allele Detail

Summary

• Symbol: Htr3a^tm1Ckn
• Name: 5-hydroxytryptamine (serotonin) receptor 3A; targeted mutation 1, Debra A Cockayne
• MGI ID: MGI:3045973
• Synonyms: 5HT3A^vs
• Gene: Htr3a Location: Chr9:48810513-48822399 bp, - strand Genetic Position: Chr9, 26.53 cM
• Alliance: Htr3a^tm1Ckn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90942
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A point mutation was introduced into exon 7, resulting in a valine to serine substitution at residue 290 (V290S). Exon 7 encodes the M2 channel domain. Cre-mediated recombination removed a floxed neo that was inserted into intron 5. PCR confirmed recombination and RT-PCR indicated reduced levels of mRNA in mutants. (J:90942)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Htr3a Mutation: 33 strains or lines available

References

• Original: J:90942 Bhattacharya A, et al., Uropathic observations in mice expressing a constitutively active point mutation in the 5-HT3A receptor subunit. J Neurosci. 2004 Jun 16;24(24):5537-48
• All: 1 reference(s)