Tg(SFFV-Sfpi1)R36Fmg
Transgene Detail

Summary

• Symbol: Tg(SFFV-Sfpi1)R36Fmg
• Name: transgene insertion R36, Francois Moreau-Gachelin
• MGI ID: MGI:3045632
• Synonyms: SFFV LTR spi-1, SFFV-LTR-spi-1, spi-1-Tg
• Transgene: Tg(SFFV-Sfpi1)R36Fmg Location: unknown
• Alliance: Tg(SFFV-Sfpi1)R36Fmg page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(SFFV-Sfpi1)R36Fmg expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Spi1 (MGI:98282)

• Mutation details: The transgene comprises a Sfpi1 minigene under transcriptional control of the long terminal repeat (LTR) of the spleen focus-forming virus (SFFV) responsible for Friend erythroleukemia. The minigene was composed of genomic DNA fragments containing exons 1 and 2 and the 5' end of exon 3, each exon flanked by some contiguous DNA (100 - 500 base pairs) from its neighboring intron(s), joined to a partial cDNA derived from the remainder of exon 3 plus exons 4 and 5. This minigene, which includes the endogeous translation initiation and termination codons and polyadenylation signal, was ligated to a fragment of the SFFV LTR that included U3, R and 20 base pairs of U5. (J:32525)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:32525 Moreau-Gachelin F, et al., Spi-1/PU.1 transgenic mice develop multistep erythroleukemias. Mol Cell Biol. 1996 May;16(5):2453-63
• All: 9 reference(s)