Kit^tm3.1Bsm
Targeted Allele Detail

Summary

• Symbol: Kit^tm3.1Bsm
• Name: KIT proto-oncogene receptor tyrosine kinase; targeted mutation 3.1, Peter Besmer
• MGI ID: MGI:3044953
• Synonyms: Kit^Y567F
• Gene: Kit Location: Chr5:75735647-75817382 bp, + strand Genetic Position: Chr5, 39.55 cM
• Alliance: Kit^tm3.1Bsm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90485
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Nucleotide substitutions
• Mutation details: Cre-mediated recombination removed the floxed neo in Kit^tm3Bsm, leaving a single loxP site in intron 9 and the tyrosine 567 to phenylalanine mutation. Flow cytometry indicated that receptor levels were reduced to 50-60% of normal levels, and Rnase protection assay showed that transcripts are reduced to 50-60% of normal levels, putatively indicating that the remaining lox site and flanking sequences in intron 9 of the mutant affects RNA transcription and/or splicing. (J:90485)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kit Mutation: 177 strains or lines available

References

• Original: J:90485 Agosti V, et al., Critical Role for Kit-mediated Src Kinase But Not PI 3-Kinase Signaling in Pro T and Pro B Cell Development. J Exp Med. 2004 Mar 15;199(6):867-78
• All: 2 reference(s)