Cd274^tm1Lpc
Targeted Allele Detail

Summary

• Symbol: Cd274^tm1Lpc
• Name: CD274 antigen; targeted mutation 1, Lieping Chen
• MGI ID: MGI:3043876
• Synonyms: B7-H1^-, B7H1^-, Cd274-Ko, Cd274^null, Pdl1^-
• Gene: Cd274 Location: Chr19:29344855-29365495 bp, + strand Genetic Position: Chr19, 23.88 cM, cytoband C2
• Alliance: Cd274^tm1Lpc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89781
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1, encoding the start codon and signal peptide, and a portion of exon 2, encoding the IgV domain, were replaced with a neo cassette inserted by homologous recombination. Protein was undetected on the surface of homozygous mutant CD4⁺ T cells via flow cytometric analysis. Western blot analysis further confirmed the absence of normal protein in homozygous mutant mice. (J:89781)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd274 Mutation: 36 strains or lines available

References

• Original: J:89781 Dong H, et al., B7-H1 determines accumulation and deletion of intrahepatic CD8(+) T lymphocytes. Immunity. 2004 Mar;20(3):327-36
• All: 64 reference(s)