Gjb2^tm1Mony
Targeted Allele Detail

Summary

• Symbol: Gjb2^tm1Mony
• Name: gap junction protein, beta 2; targeted mutation 1, Hannah Monyer
• MGI ID: MGI:3043744
• Synonyms: Cx26^lacZ
• Gene: Gjb2 Location: Chr14:57336059-57342159 bp, - strand Genetic Position: Chr14, 30.1 cM, cytoband D1-E1
• Alliance: Gjb2^tm1Mony page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89907
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Hypomorph, Reporter)
• Mutation: Insertion
• Mutation details: A cassette containing IRES-lacZ and neo was inserted immediately downstream of the stop codon in exon 2. While undetectable by Northern blot analysis, transcript was identified by RT-PCR analysis indicating the amount of message in homozygous mutant mice to be severely reduced relative to that of wild-type. The inserted lacZ gene was expressed. (J:89907)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gjb2 Mutation: 21 strains or lines available

References

• Original: J:89907 Filippov MA, et al., A reporter allele for investigating connexin 26 gene expression in the mouse brain. Eur J Neurosci. 2003 Dec;18(12):3183-92
• All: 1 reference(s)