Fem1c^{Gt(ROSABetageo)3Sor}
Gene trapped Allele Detail

Summary

• Symbol: Fem1c^{Gt(ROSABetageo)3Sor}
• Name: fem 1 homolog c; gene trap 3, Philippe Soriano
• MGI ID: MGI:3043637
• Synonyms: Fem1c^R3
• Gene: Fem1c Location: Chr18:46637673-46659038 bp, - strand Genetic Position: Chr18, 24.62 cM
• Alliance: Fem1c^{Gt(ROSABetageo)3Sor} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89876
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: The endogenous gene was disrupted by the insertion of the ROSA vector containing a beta-galactosidase/neo fusion protein into intron 1. While sequence analysis of RT-PCR products showed splicing between exon 1 and the ROSA cassette and did not detect transcript in which exon 1 was spliced to exon 2, transcript containing exons 2 and 3 (encompassing the entire coding region) was detected in some tissues, including testes and brain, but this latter transcript was not detected in eye, kidney, or cardiac muscle tissue. (J:89876)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fem1c Mutation: 30 strains or lines available

References

• Original: J:89876 Schlamp CL, et al., Insertion of the beta Geo promoter trap into the Fem1c gene of ROSA3 mice. Mol Cell Biol. 2004 May;24(9):3794-803
• All: 2 reference(s)