Trp53^tm3.1Tyj
Targeted Allele Detail

Summary

• Symbol: Trp53^tm3.1Tyj
• Name: transformation related protein 53; targeted mutation 3.1, Tyler Jacks
• MGI ID: MGI:3039266
• Synonyms: p53^H, p53^R270H, p53 R270H, Trp53^R270H
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm3.1Tyj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95316
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: The loxP site flanked STOP cassette was removed from Trp53^tm3Tyj via Cre-mediated recombination, allowing for the expression of the p.R270H missense mutation in exon 8. Quantitative RT-PCR confirmed that the point mutation allele was expressed at a level comparable to wild-type. (J:126915)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Trp53^tm3.1Tyj

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 232 strains or lines available

References

• Original: J:95316 Olive KP, et al., Mutant p53 gain of function in two mouse models of Li-Fraumeni syndrome. Cell. 2004 Dec 17;119(6):847-60
• All: 22 reference(s)