Endog/D2Wsu81e^tm1Mxu
Targeted Allele Detail

Summary

• Symbol: Endog/D2Wsu81e^tm1Mxu
• Name: endonuclease G; targeted mutation 1, Ming Xu
• MGI ID: MGI:3034030
• Synonyms: EndoG^-, Endog^tm1Mxu
• Gene: Endog Location: Chr2:30061525-30064057 bp, + strand Genetic Position: Chr2, 21.29 cM, cytoband B
• Alliance: Endog/D2Wsu81e^tm1Mxu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88144
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: The majority of the 3 exons of the Endog and 5 of 10 exons of D2Wsu81e (which overlaps exon 3 of Endog) were replaced with a neomycin selection cassette inserted by homologous recombination. Reduced ENDOG protein levels were detected by Western blot analysis of protein extracts obtained from heterozygous MEFs and hearts. (J:88144, J:94752)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Endog Mutation: 12 strains or lines available

References

• Original: J:88144 Zhang J, et al., Endonuclease G is required for early embryogenesis and normal apoptosis in mice. Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15782-7
• All: 2 reference(s)