Procr^tm1Fjc
Targeted Allele Detail

Summary

• Symbol: Procr^tm1Fjc
• Name: protein C receptor, endothelial; targeted mutation 1, Francis J Castellino
• MGI ID: MGI:3033747
• Synonyms: EPCR^delta, EPCR^low, Procr^delta
• Gene: Procr Location: Chr2:155593037-155597391 bp, + strand Genetic Position: Chr2, 77.26 cM, cytoband H1-3
• Alliance: Procr^tm1Fjc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80955
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Insertion
• Mutation details: A severely hypomorphic allele was generated after aberrant recombination resulted in the insertion of the entire targeting vector, along with 1.2 kb of plasmid backbone, 304 bp upstream of the endogenous locus. Quantitative RT-PCR showed Procr transcript levels to be less than 5% those of wild-type in a variety of organs (with the exception of the thymus in which levels were ~10% those of wild-type) and ~1% those of wild-type in embryos at E9.5 and E12.5. The vector included 4.4 kb of 5' Procr sequence, a PGK-neo cassette with polyA signal, 3.5 kb of 3' Procr sequence, and a PGK-cda (cytosine deaminase) with polyA signal. (J:80955)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Procr Mutation: 15 strains or lines available

References

• Original: J:80955 Castellino FJ, et al., Mice with a severe deficiency of the endothelial protein C receptor gene develop, survive, and reproduce normally, and do not present with enhanced arterial thrombosis after challenge. Thromb Haemost. 2002 Sep;88(3):462-72
• All: 13 reference(s)