Pparg^tm1Auw
Targeted Allele Detail

Summary

• Symbol: Pparg^tm1Auw
• Name: peroxisome proliferator activated receptor gamma; targeted mutation 1, Johan Auwerx
• MGI ID: MGI:2683406
• Synonyms: PPARgamma^hyp
• Gene: Pparg Location: Chr6:115337912-115467360 bp, + strand Genetic Position: Chr6, 53.41 cM, cytoband E3-F1
• Alliance: Pparg^tm1Auw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86705
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Nucleotide substitutions
• Mutation details: A hypomorphic allele was generated by inserting an frt flanked neomycin selection cassette in the intron following exon B. The targeting vector also included a proline to alaline missense mutation at codon 12 (P12A) and loxP sites flanking exon B and the neo cassette. mRNA corresponding to both alternatively spliced transcripts 1 and 2 were significantly reduced in homozygous mutant white adipose tissue. While variant 2 transcript was nearly undetected in homozygous mutant brown adipose tissue, Variant 1 was significantly increased relative to wild-type. (J:86705)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pparg Mutation: 39 strains or lines available

References

• Original: J:86705 Koutnikova H, et al., Compensation by the muscle limits the metabolic consequences of lipodystrophy in PPAR gamma hypomorphic mice. Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):14457-62
• All: 3 reference(s)