L1cam^tm1Fmo
Targeted Allele Detail

Summary

• Symbol: L1cam^tm1Fmo
• Name: L1 cell adhesion molecule; targeted mutation 1, Fabio Morellini
• MGI ID: MGI:2682895
• Gene: L1cam Location: ChrX:72897384-72924843 bp, - strand Genetic Position: ChrX, 37.43 cM, cytoband A6-B
• Alliance: L1cam^tm1Fmo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86626
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: An frt flanked PGK-neomycin resistance cassette was inserted downstream of exon 5. In addition, exon 5 and the inserted neomycin gene were flanked by 2 loxP sites. Successfully targeted ES cells were transiently transfected with flp-recombinase to remove the neomycin gene. Neither the loxP sequences nor the remaining frt sequence had an adverse affect on gene expression as determined by Western blot analysis. (J:86626)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any L1cam Mutation: 12 strains or lines available

References

• Original: J:86626 Law JW, et al., Decreased anxiety, altered place learning, and increased CA1 basal excitatory synaptic transmission in mice with conditional ablation of the neural cell adhesion molecule L1. J Neurosci. 2003 Nov 12;23(32):10419-32
• All: 4 reference(s)