Ets2^tm2Rgo
Targeted Allele Detail

Summary

• Symbol: Ets2^tm2Rgo
• Name: E26 avian leukemia oncogene 2, 3' domain; targeted mutation 2, Robert G Oshima
• MGI ID: MGI:2682649
• Synonyms: Ets2^tm1Osh, Ets2^tmA72Osh, Ets^A72
• Gene: Ets2 Location: Chr16:95503274-95522093 bp, + strand Genetic Position: Chr16, 56.64 cM, cytoband C3-qter
• Alliance: Ets2^tm2Rgo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86530
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The ACT codon for threonine at amino acid residue 72 was changed to a GCT alanine codon. At the same time, the CCC codon for amino acid residue 73 was changed to CCA which eliminated a Pst 1 restriction site but did not have any other effect. Finally, a floxed neomycin gene was inserted in intron 4. The neomycin resistance gene was removed in vivo by mating to mice expressing Cre recombinase from the protamine promoter in the male germ line. Production and turnover of the modified protein were similar to unmodified protein. (J:86530)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ets2 Mutation: 37 strains or lines available

References

• Original: J:86530 Man AK, et al., Ets2-dependent stromal regulation of mouse mammary tumors. Mol Cell Biol. 2003 Dec;23(23):8614-25
• All: 7 reference(s)