Rag1^tm1Fwa
Targeted Allele Detail

Summary

• Symbol: Rag1^tm1Fwa
• Name: recombination activating 1; targeted mutation 1, Frederick W Alt
• MGI ID: MGI:2681693
• Synonyms: R1core, RAG1^c
• Gene: Rag1 Location: Chr2:101468627-101479846 bp, - strand Genetic Position: Chr2, 53.88 cM
• Alliance: Rag1^tm1Fwa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86464
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The Rag1 coding sequence was replaced with a truncated core Rag-1 sequence followed by a floxed neomycin resistance gene. The neomycin gene was then removed from properly targeted ES cells by transient transfection with a plasmid based Cre recombinase. Core Rag1 was expressed at levels comparable to that found for intact protein. (J:86464)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rag1 Mutation: 120 strains or lines available

References

• Original: J:86464 Dudley DD, et al., Impaired V(D)J recombination and lymphocyte development in core RAG1-expressing mice. J Exp Med. 2003 Nov 3;198(9):1439-50
• All: 35 reference(s)