Foxn1^tm1Nrm
Targeted Allele Detail

Summary

• Symbol: Foxn1^tm1Nrm
• Name: forkhead box N1; targeted mutation 1, Nancy R Manley
• MGI ID: MGI:2680674
• Synonyms: Foxn1^delta
• Gene: Foxn1 Location: Chr11:78248403-78277384 bp, - strand Genetic Position: Chr11, 46.74 cM
• Alliance: Foxn1^tm1Nrm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86255
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Insertion
• Mutation details: The insertion of sequence encoding GFP into exon 3 resulted in the generation of a hypomorphic allele lacking exon 3. Though the floxed neo cassette used for selection was excised, inverted repeats at the insertion site of the remaining loxP site may be the reason for the aberrant splicing. Mutant transcript was detected by Northern blot, RT-PCR, and RNase protection. Sequence analysis of the RT-PCR products showed that exon 2 spliced directly to exon 4, producing a transcript lacking exon 3 similar to a splice variant found at low levels in wild-type mice. The transcript putatively encodes protein lacking 154 residues from the N-terminal domain, but containing the nuclear localization signal and DNA-binding and C-terminal acidic activation domains. (J:86255)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Foxn1 Mutation: 106 strains or lines available

References

• Original: J:86255 Su DM, et al., A domain of Foxn1 required for crosstalk-dependent thymic epithelial cell differentiation. Nat Immunol. 2003 Nov;4(11):1128-35
• All: 5 reference(s)