Trp53^tm1Xu
Targeted Allele Detail

Summary

• Symbol: Trp53^tm1Xu
• Name: transformation related protein 53; targeted mutation 1, Yang Xu
• MGI ID: MGI:2680218
• Synonyms: p53^S18A
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm1Xu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86185
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: Site directed mutagenesis within exon 2 generated a missense mutation (AG>GC) at codon 18 resulting in the substitution of serine with alanine (p.S18A). Serine 18 corresponds with human serine 15 of TP53 and this mutation prevents phosphorylation of the residue in the encoded peptide. A single loxP site remained in intron 3 after the inserted loxP site flanked neomycin resistance gene cassette was excised via Cre-mediated recombination. (J:86520)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 232 strains or lines available

References

• Original: J:86185 Chao C, et al., Cell type- and promoter-specific roles of Ser18 phosphorylation in regulating p53 responses. J Biol Chem. 2003 Oct 17;278(42):41028-33
• All: 4 reference(s)