Tg(SLC18A3-cre)KMisa
Transgene Detail

Summary

• Symbol: Tg(SLC18A3-cre)KMisa
• Name: transgene insertion K, Hidemi Misawa
• MGI ID: MGI:2679332
• Synonyms: VAChT.Cre.Fast, VAChT-Cre-K
• Transgene: Tg(SLC18A3-cre)KMisa Location: unknown
• Alliance: Tg(SLC18A3-cre)KMisa page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Mutation details: The transgenic construct consisted of an 11.3 kb fragment of the human cholinergic gene locus, containing the first 5 exons of CHAT and the first coding exon of SLC18A3 (located in intron 1 of CHAT). A cassette containing cre followed by IRES-EGFP was inserted at the start codon of the SLC18A3 gene. EGFP expression was undetected. The 11.3 kb fragment included various promoter elements including a distal enhancer, RE1/NRSE, and NGFRE. Founder mice contained approximately 20 copies. Expression of cre recombinase by the human SLC18A3 promoter began at postnatal day 7 and was restricted to a subset of cholinergic neurons in the somatomotor nuclei and medial habenular nucleus. Maximal levels of expression were reached by 5 weeks of age,earlier onset than in the Tg(SLC18a3-cre)AMisa line. Transgenic expression was not observed in viscertomotor or other central or peripheral cholinergic neurons. (J:86160)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	SLC18A3 (human) Summary of all recombinase alleles driven by SLC18A3. Comparative matrix view of recombinase activities Alliance human SLC18A3 gene page

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:86160 Misawa H, et al., VAChT-Cre.Fast and VAChT-Cre.Slow: postnatal expression of Cre recombinase in somatomotor neurons with different onset. Genesis. 2003 Sep;37(1):44-50
• All: 14 reference(s)