Plk2^tm1Rle
Targeted Allele Detail

Summary

• Symbol: Plk2^tm1Rle
• Name: polo like kinase 2; targeted mutation 1, Raymond L Erikson
• MGI ID: MGI:2678106
• Synonyms: Plk2^-
• Gene: Plk2 Location: Chr13:110531580-110537378 bp, + strand Genetic Position: Chr13, 61.97 cM, cytoband D2.1
• Alliance: Plk2^tm1Rle page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85756
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 750 bp fragment encompassing a portion of exon 1 and all of exon 2 was replaced with a neomycin selection cassette inserted by homologous recombination. Truncated transcript consisting of the neo cassette and portions of intron 2 and exon 3. The mutant transcript lacks sequence encoding the first 2 kinase domains which include the conserved putative ATP-binding pocket. Protein was undetected by Western blot analysis of homozygous mutant MEFs. (J:85756)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Plk2 Mutation: 32 strains or lines available

References

• Original: J:85756 Ma S, et al., Role of Plk2 (Snk) in mouse development and cell proliferation. Mol Cell Biol. 2003 Oct;23(19):6936-43
• All: 1 reference(s)