Cdkn2a^tm1Nesh
Targeted Allele Detail

Summary

• Symbol: Cdkn2a^tm1Nesh
• Name: cyclin dependent kinase inhibitor 2A; targeted mutation 1, Norman E Sharpless
• MGI ID: MGI:2677722
• Synonyms: Arf^-, P19^ARF-
• Gene: Cdkn2a Location: Chr4:89192710-89212856 bp, - strand Genetic Position: Chr4, 42.15 cM, cytoband C3-C6
• Alliance: Cdkn2a^tm1Nesh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88365
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A premature stop codon was generated in exon 1 beta, unique to P19^ARF, by cre-mediated excision of a floxed neomycin cassette, leaving the cis-regulatory elements of both P19^ARF and P16^INK4 intact. Loss of P19^ARF and presence of P16^INK4 was confirmed by western blot analysis of homozygous mutant MEFs. (J:88365)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Cdkn2a^tm1Nesh

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cdkn2a Mutation: 62 strains or lines available

References

• Original: J:88365 Sharpless NE, et al., The differential impact of p16(INK4a) or p19(ARF) deficiency on cell growth and tumorigenesis. Oncogene. 2004 Jan 15;23(2):379-85
• All: 11 reference(s)