Sirt1^tm1Fwa
Targeted Allele Detail

Summary

• Symbol: Sirt1^tm1Fwa
• Name: sirtuin 1; targeted mutation 1, Frederick W Alt
• MGI ID: MGI:2677354
• Synonyms: SIRT1^deltaex4
• Gene: Sirt1 Location: Chr10:63154784-63174814 bp, - strand Genetic Position: Chr10, 32.57 cM
• Alliance: Sirt1^tm1Fwa page

Developmental abnormalities in Sirt1^tm1Fwa/Sirt1^tm1Fwa mice.

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85542
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neomycin resistance cassette in reverse orientation was placed at the 3' end of intron 3. An additional loxP site was inserted just downstream of exon 4. In the resulting ES cells, exon 4, which encodes 51 amino acid residues of the conserved catalytic domain, is flanked by loxP sites. An AdenoCre construct was used to remove the neomycin selection cassette and exon 4. Corresponding protein was detected by Western blot analysis. These mutant ES cells, prior to cre recombination, were also used in the generation of Sirt1^tm3Fwa mice. (J:85542)

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sirt1 Mutation: 78 strains or lines available

References

• Original: J:85542 Cheng HL, et al., Developmental defects and p53 hyperacetylation in Sir2 homolog (SIRT1)-deficient mice. Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10794-9
• All: 20 reference(s)