Igf2^tm1Wrk
Targeted Allele Detail

Summary

• Symbol: Igf2^tm1Wrk
• Name: insulin-like growth factor 2; targeted mutation 1, Wolf Reik
• MGI ID: MGI:2677155
• Synonyms: DeltaDMR1-U2, Igf2P0-, Igf2^tm1Wrk-pat, P0
• Gene: Igf2 Location: Chr7:142204503-142220553 bp, - strand Genetic Position: Chr7, 87.99 cM
• Alliance: Igf2^tm1Wrk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:65001
• Parent Cell Line: CGR8 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutation: Insertion
• Mutation details: Sequence encompassing the second placenta-specific exon (U2) and the first differentially methylated region (DMR1 or Rr15), located 3 kb upstream of the first fetal promoter (P1), was replaced by a single loxP site following cre-mediated recombination. In contrast to the wild-type allele, which is silenced soon after birth, both maternally and paternally transmitted alleles continued to be transcribed postnatally. In addition to the loss of imprinting, the deletion resulted in the ablation of placenta-specific transcript. Transcript was undetected by in situ hybridization of placentae from heterozygous mice carrying a paternally transcribed mutant allele. (J:65001)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igf2 Mutation: 25 strains or lines available

References

• Original: J:65001 Constancia M, et al., Deletion of a silencer element in igf2 results in loss of imprinting independent of H19. Nat Genet. 2000 Oct;26(2):203-6
• All: 14 reference(s)