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Gja1tm5(cre/ERT)Kwi
Targeted Allele Detail
Summary
Symbol: Gja1tm5(cre/ERT)Kwi
Name: gap junction protein, alpha 1; targeted mutation 5, Klaus Willecke
MGI ID: MGI:2676327
Synonyms: Cx43Cre-ER(T), Gja1tm2(cre/ESR1)Kwi, Gja1tm5(cre/ESR1)Kwi
Gene: Gja1  Location: Chr10:56253297-56266519 bp, + strand  Genetic Position: Chr10, 28.64 cM
Alliance: Gja1tm5(cre/ERT)Kwi page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:85065
Parent Cell Line:  HM-1 (ES Cell)
Strain of Origin:  129P2/OlaHsd-Hprt1b-m3
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutations:    Insertion, Intragenic deletion
 
Gja1tm5(cre/ERT)Kwi expression driven by 1 gene
 
Mutation detailsThe coding region was replaced with a construct encoding a tamoxifen dependent cre recombinase. The cre recombinase coding region was joined to sequence encoding the ligand binding domain of the human estrogen receptor with a glycine to arginine missense mutation at position 521. This substitution enables the domain to bind to tamoxifen while ablating the estradiol binding ability. Tamoxifen treatment permits the translocation of the cre hybrid protein to the nucleus. (J:85065)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Gja1 (mouse)
Summary of all recombinase alleles driven by Gja1.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gja1 Mutation:  59 strains or lines available
References
Original:  J:85065 Kretz M, et al., Altered connexin expression and wound healing in the epidermis of connexin-deficient mice. J Cell Sci. 2003 Aug 15;116(Pt 16):3443-52
All:  18 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
07/02/2024
MGI 6.13
The Jackson Laboratory