Spmap2^tm1Wen
Targeted Allele Detail

Summary

• Symbol: Spmap2^tm1Wen
• Name: sperm microtubule associated protein 2; targeted mutation 1, Wolfgang Engel
• MGI ID: MGI:2674963
• Synonyms: Th14^-
• Gene: Spmap2 Location: Chr10:79412311-79422970 bp, - strand Genetic Position: Chr10, 39.72 cM, cytoband B5-C1
• Alliance: Spmap2^tm1Wen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85175
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Disruption caused by insertion of vector
• Mutation details: Exons 4, 5, 6, and 7 were deleted by homologous recombination of neo cassette. The targeted region encoded amino acids 137 through 376 of the C-terminal. Mutant transcript consisting of exons 1 and 2 spliced to the neo cassette was detected by RT-PCR analysis. Immunotstaining revealed that mutant protein was produced and that it localized to the cytosol of round spermatids in contrast to the wild-type protein which predominantly localizes to the nucleus. (J:85175)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spmap2 Mutation: 17 strains or lines available

References

• Original: J:85175 Mannan AU, et al., Male mice lacking the Theg (testicular haploid expressed gene) protein undergo normal spermatogenesis and are fertile. Biol Reprod. 2003 Sep;69(3):788-96
• All: 1 reference(s)