Tg(HBB)4Cos
Transgene Detail

Summary

• Symbol: Tg(HBB)4Cos
• Name: transgene insertion 4, Franklin Costantini
• MGI ID: MGI:2673816
• Transgene: Tg(HBB)4Cos Location: unknown
• Alliance: Tg(HBB)4Cos page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(HBB)4Cos expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  rabbit, European	HBB2 (100009084)

• Mutation details: The transgene includes the rabbit adult beta hemoglobin gene and a beta-2 pseudogene. in situ hybridization studies of metaphase spreads from the founder mouse chromosomes detected one transgene insertion site on Chromosome 3 and another on Chromosome 12. Southern blot analyses of G1 mice estimated 3-4 transgene copies per cell on Chr 3 and 15-25 copies on Chr 12. 3' S1 nuclease protection assays detected polyadenylated rabbit transcript in skeletal muscle (20x), brain, testis, and lung (1x), but not in erythrocytes from a G2 mouse carrying the transgene. Further 3' S1 nuclease protection studies of G2 and G3 mice carrying the transgene indicate that the rabbit transcripts in muscle have the correct 5' terminus.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:71318 Lacy E, et al., A foreign beta-globin gene in transgenic mice: integration at abnormal chromosomal positions and expression in inappropriate tissues. Cell. 1983 Sep;34(2):343-58
• All: 1 reference(s)