Pdgfb^tm3Cbet
Targeted Allele Detail

Summary

• Symbol: Pdgfb^tm3Cbet
• Name: platelet derived growth factor, B polypeptide; targeted mutation 3, Christer Betsholtz
• MGI ID: MGI:2670572
• Gene: Pdgfb Location: Chr15:79880075-79899178 bp, - strand Genetic Position: Chr15, 37.85 cM, cytoband E
• Alliance: Pdgfb^tm3Cbet page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:84701
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A stop codon was inserted into exon 6 via homologous recombination of a targeting vector which also included a loxP flanked neo cassette in intron 5. The insertion of the stop codon precludes the translation of the retention moftif. Mice carrying this allele were bred with transgenic mice expressing cre recombinase to generate Pdgfb^tm3.1Cbet. (J:84701)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdgfb Mutation: 15 strains or lines available

References

• Original: J:84701 Lindblom P, et al., Endothelial PDGF-B retention is required for proper investment of pericytes in the microvessel wall. Genes Dev. 2003 Aug 1;17(15):1835-40
• All: 3 reference(s)