Mt2^tm1Rch
Targeted Allele Detail

Summary

• Symbol: Mt2^tm1Rch
• Name: metallothionein 2; targeted mutation 1, Anna E Michalska
• MGI ID: MGI:2668455
• Synonyms: MT-II -, MT-null
• Gene: Mt2 Location: Chr8:94899292-94900196 bp, + strand Genetic Position: Chr8, 46.32 cM
• Alliance: Mt2^tm1Rch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:14416
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Both metallothionein I and II were disrupted using the same targeting construct. The second exon of metallothionein II had an insertion of the pMC1NeoPA selection marker at a position 30bp downstream of the start codon. Northern blot analysis of liver mRNA from homozygotes in which the gene was induced by intraperitoneal injection of copper demonstrated that transcription was prevented. Further, no protein product was detected in the livers of these animals. (J:14416)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mt2 Mutation: 8 strains or lines available

References

• Original: J:14416 Michalska AE, et al., Targeting and germ-line transmission of a null mutation at the metallothionein I and II loci in mouse. Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8088-92
• All: 26 reference(s)