Tg(PAH-INSR)1Rjv
Transgene Detail

Summary

• Symbol: Tg(PAH-INSR)1Rjv
• Name: transgene insertion 1, Rajiv L Joshi
• MGI ID: MGI:2665339
• Synonyms: PAH-IRdelta
• Transgene: Tg(PAH-INSR)1Rjv Location: unknown
• Alliance: Tg(PAH-INSR)1Rjv page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(PAH-INSR)1Rjv expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  INSR (3643)

  Insr	(MGI:96575)

• Mutation details: The transgene consists of a human PAH promoter and a sequence endcoding a truncated and activated from of human INSR. The mutant INSR contains the first 12 amino acids of the alpha subunit fused to 20% of the C-terminal portion of the alpha subunit and the entire beta subunit. Strong transgene expression in the liver (approximately 6.5-fold higher than endogenous mouse mRNA) was confirmed by RT-PCR and Western blot. Weak transgene expression was also observed in kidneys and testis. (J:80773)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:80773 Baudry A, et al., Partial rescue of insulin receptor-deficient mice by transgenic complementation with an activated insulin receptor in the liver. Gene. 2002 Oct 16;299(1-2):219-25
• All: 1 reference(s)