Apex1^tm2Cur
Targeted Allele Detail

Summary

• Symbol: Apex1^tm2Cur
• Name: apurinic/apyrimidinic endonuclease 1; targeted mutation 2, Tom Curran
• MGI ID: MGI:2664368
• Synonyms: ref-1^C64A
• Gene: Apex1 Location: Chr14:51162425-51164596 bp, + strand Genetic Position: Chr14, 26.3 cM, cytoband C3
• Alliance: Apex1^tm2Cur page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83750
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A cysteine to alanine missense mutation was generated at codon 64 (C64A) in exon 2 by site directed mutagenesis. An artifactual loxP site remained in intron 2 after the floxed neo selection cassette was removed by in vitro cre mediated recombination. The incorporation of the missense mutation was confirmed by sequence analysis and protein levels similar to those of wild-type were detected by Western blot anlaysis of homozygous mutant mice. (J:83750)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Apex1 Mutation: 16 strains or lines available

References

• Original: J:83750 Ordway JM, et al., Cysteine 64 of Ref-1 is not essential for redox regulation of AP-1 DNA binding. Mol Cell Biol. 2003 Jun;23(12):4257-66
• All: 1 reference(s)