Rtn4^tm2Matl
Targeted Allele Detail

Summary

• Symbol: Rtn4^tm2Matl
• Name: reticulon 4; targeted mutation 2, Marc Tessier-Lavigne
• MGI ID: MGI:2661167
• Synonyms: Nogo-A/B/C
• Gene: Rtn4 Location: Chr11:29642947-29694331 bp, + strand Genetic Position: Chr11, 16.79 cM
• Alliance: Rtn4^tm2Matl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83192
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The first of 6 exons common to isoforms A, B, and C was replaced with a floxed neo cassette inserted by homologous recombination. The deleted exon encodes the first transmembrane domain and a portion of an extracellular loop. While a shortened C transcript was identified by Northern blot analysis, A and B transcripts were undetected in homozygous mutant mice. RT-PCR and sequence analysis showed the aberrant transcript to lack the neo cassette (as well as the 66 codons it had replaced) and to contain a frameshift mutation after the first 11 codons. (J:83192)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rtn4 Mutation: 130 strains or lines available

References

• Original: J:83192 Zheng B, et al., Lack of enhanced spinal regeneration in Nogo-deficient mice. Neuron. 2003 Apr 24;38(2):213-24
• All: 3 reference(s)