Gusb^tm4Sly
Targeted Allele Detail

Summary

• Symbol: Gusb^tm4Sly
• Name: glucuronidase, beta; targeted mutation 4, William S Sly
• MGI ID: MGI:2661042
• Synonyms: Gus^{tm(hE540A.mE536A)Sly}
• Gene: Gusb Location: Chr5:130017852-130031890 bp, - strand Genetic Position: Chr5, 68.32 cM
• Alliance: Gusb^tm4Sly page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83169
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Molecular techniques were employed to generate an allele containing E536A in exon 10 and a cDNA sequence encoding an inactive form of human GUS in intron 9. The human cDNA contained the E540A missense mutation. Inactive forms of both human and murine protein were produced, resulting in mutant mice that possess immunologic tolerance to both human and murine wild-type enzymes. (J:83169)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gusb Mutation: 43 strains or lines available

References

• Original: J:83169 Tomatsu S, et al., Production of MPS VII mouse (Gus(tm(hE540A.mE536A)Sly)) doubly tolerant to human and mouse beta-glucuronidase. Hum Mol Genet. 2003 May 1;12(9):961-73
• All: 2 reference(s)