Gria1^tm1Rlh
Targeted Allele Detail

Summary

• Symbol: Gria1^tm1Rlh
• Name: glutamate receptor, ionotropic, AMPA1 (alpha 1); targeted mutation 1, Richard L Huganir
• MGI ID: MGI:2653986
• Synonyms: GR1AA
• Gene: Gria1 Location: Chr11:56902342-57221070 bp, + strand Genetic Position: Chr11, 34.51 cM
• Alliance: Gria1^tm1Rlh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82443
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Alanine amino acid substitutions were introduced to phosphorylation sites serine 831 (S831A) and serine 845 (S845A) in exon 17 of the gene. A floxed neomycin resistance cassette was inserted upstream of exon 17 for positive selection. Mutant animals were crossed to cre deleter strain Tg(CMV-cre)1Nagy to removed the floxed neo cassette, leaving behind the 2 amino acid substitutions. The targeted mutation was confirmed using immunoblot with phosphorylation site-specific antibodies. (J:82443)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gria1 Mutation: 62 strains or lines available

References

• Original: J:82443 Lee HK, et al., Phosphorylation of the AMPA receptor GluR1 subunit is required for synaptic plasticity and retention of spatial memory. Cell. 2003 Mar 7;112(5):631-43
• All: 13 reference(s)