Ppp1r3a^tm1Ptwc
Targeted Allele Detail

Summary

• Symbol: Ppp1r3a^tm1Ptwc
• Name: protein phosphatase 1, regulatory subunit 3A; targeted mutation 1, Patricia T W Cohen
• MGI ID: MGI:2653915
• Synonyms: Gm
• Gene: Ppp1r3a Location: Chr6:14713976-14755273 bp, - strand Genetic Position: Chr6, 6.31 cM, cytoband A2
• Alliance: Ppp1r3a^tm1Ptwc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82422
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A single loxP site was left in place of exon 1 after the insertion and subsequent removal of a floxed neo cassette by homologous recombination and in vitro cre mediated excision. The deleted region encoded the start codon as well as the PP1 and glycogen-binding domains. Western blot analysis of homozygous mutant skeletal muscle indicated an absence of protein. (J:82422)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ppp1r3a Mutation: 57 strains or lines available

References

• Original: J:82422 Delibegovic M, et al., Disruption of the striated muscle glycogen targeting subunit PPP1R3A of protein phosphatase 1 leads to increased weight gain, fat deposition, and development of insulin resistance. Diabetes. 2003 Mar;52(3):596-604
• All: 2 reference(s)