Ly6a^tm1Grau
Targeted Allele Detail

Summary

• Symbol: Ly6a^tm1Grau
• Name: lymphocyte antigen 6 family member A; targeted mutation 1, Timothy A Graubert
• MGI ID: MGI:2651374
• Synonyms: Sca-1^EGFP
• Gene: Ly6a Location: Chr15:74866726-74869880 bp, - strand Genetic Position: Chr15, 34.29 cM
• Alliance: Ly6a^tm1Grau page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82052
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A cDNA sequence encoding EGFP was inserted in place of the start codon in exon 2 of the endogenous locus. A single loxP site remained between the EGFP sequence and the coding region of exon 2. Expression of EGFP was detected in all hempatopoietic tissues of heterozygous mutant mice. The insertion did not disrupt the expression patterns of neighboring genes, Ly6i and Ly6e. (J:82052)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ly6a Mutation: 20 strains or lines available

References

• Original: J:82052 Hanson P, et al., Enhanced green fluorescent protein targeted to the Sca-1 (Ly-6A) locus in transgenic mice results in efficient marking of hematopoietic stem cells in vivo. Exp Hematol. 2003 Feb;31(2):159-67
• All: 7 reference(s)