Met^tm4Cpo
Targeted Allele Detail

Summary

• Symbol: Met^tm4Cpo
• Name: met proto-oncogene, receptor tyrosine kinase; targeted mutation 4, Carola Ponzetta
• MGI ID: MGI:2450964
• Synonyms: Met^grb2neo+
• Gene: Met Location: Chr6:17463799-17573979 bp, + strand Genetic Position: Chr6, 7.83 cM, cytoband A2
• Alliance: Met^tm4Cpo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81793
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: A mutant human cDNA coding for the transmembrane and cytoplasmic domain of the receptor was inserted in-frame into mouse genomic sequences. The cDNA was followed by a floxed neomycin resistance cassette. A fusion protein consisting of the murine extracellular domain fused to the human transmembrane and cytoplasmic domain carrying the mutation Y1358N is produced from this allele. (J:81793)

Expression

In Mice Carrying this Mutation:

1 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Met Mutation: 81 strains or lines available

References

• Original: J:81793 Ieraci A, et al., Viable hypomorphic signaling mutant of the Met receptor reveals a role for hepatocyte growth factor in postnatal cerebellar development. Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):15200-5
• All: 1 reference(s)