Adam12^tm1Asf
Targeted Allele Detail

Summary

• Symbol: Adam12^tm1Asf
• Name: ADAM metallopeptidase domain 12; targeted mutation 1, Atsuko Sehara-Fujisawa
• MGI ID: MGI:2448241
• Synonyms: Meltrin alpha^-
• Gene: Adam12 Location: Chr7:133484928-133826826 bp, - strand Genetic Position: Chr7, 77.61 cM, cytoband F4
• Alliance: Adam12^tm1Asf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81009
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of exon 1 with a PGK-neo cassette via homologous recombination. Absence of gene expression was confirmed by RT-PCR analysis of day E14.5 homozygous mutant embryos. Primers directed against the metalloprotease, disintegrin, cysteine-rich, and cytoplasmic domains did not detect any gene expression. (J:81009)

Expression

In Mice Carrying this Mutation:	3 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adam12 Mutation: 41 strains or lines available

References

• Original: J:81009 Kurisaki T, et al., Phenotypic analysis of Meltrin alpha (ADAM12)-deficient mice: involvement of Meltrin alpha in adipogenesis and myogenesis. Mol Cell Biol. 2003 Jan;23(1):55-61
• All: 10 reference(s)