Nkx2-3^tm1Rph
Targeted Allele Detail

Summary

• Symbol: Nkx2-3^tm1Rph
• Name: NK2 homeobox 3; targeted mutation 1, Richard P Harvey
• MGI ID: MGI:2448069
• Synonyms: Nkx2-3-, Nkx2-3^lacZDeltaHD
• Gene: Nkx2-3 Location: Chr19:43600764-43604331 bp, + strand Genetic Position: Chr19, 36.67 cM
• Alliance: Nkx2-3^tm1Rph page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:64018
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 2 was disrupted by the insertion of a construct containing IRES-NLS-lacZ followed by a floxed hygromycin selection cassette. The deleted region included nucleotides 2561 through 2860 (amino acids 149-251) encoding encoding the homeodomain and a conserved NK2-specific domain. Expression of beta-galactosidase was driven by the endogenous promoter. (J:64018)

Expression

In Mice Carrying this Mutation:	26 assay results
In Structures Affected by this Mutation:	22 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nkx2-3 Mutation: 14 strains or lines available

References

• Original: J:64018 Wang CC, et al., Homeodomain factor Nkx2-3 controls regional expression of leukocyte homing coreceptor MAdCAM-1 in specialized endothelial cells of the viscera. Dev Biol. 2000 Aug 15;224(2):152-67
• All: 5 reference(s)