Apoe^tm1.1Khw
Targeted Allele Detail

Summary

• Symbol: Apoe^tm1.1Khw
• Name: apolipoprotein E; targeted mutation 1.1, Karl H Weisgraber
• MGI ID: MGI:2447431
• Synonyms: Arg-61 apoE
• Gene: Apoe Location: Chr7:19430034-19433113 bp, - strand Genetic Position: Chr7, 9.94 cM
• Alliance: Apoe^tm1.1Khw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71838
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: This allele is derived from Apoe^tm1Khw by mating mutant animals to a cre deleter strain. Resulting progeny are removed of the floxed neo cassette between exons 3 and 4 leaving behind a threonine to arginine substitution at amino acid 79 (p.T79R). This mutation allows the mouse protein to mimic human APOE4 domain interaction. Removal of the neo cassette resulted in normal levels of gene expression comparable to that of wild-type. Expression of the mutant allele was confirmed by Northern blot analysis of several tissue types. (J:71838)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Apoe Mutation: 145 strains or lines available

References

• Original: J:71838 Raffai RL, et al., Introduction of human apolipoprotein E4 'domain interaction' into mouse apolipoprotein E. Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11587-91
• All: 2 reference(s)