Tg(Prnp)C4Cwe
Transgene Detail

Summary

• Symbol: Tg(Prnp)C4Cwe
• Name: transgene insertion C4, Charles Weissmann
• MGI ID: MGI:2446641
• Synonyms: C4
• Transgene: Tg(Prnp)C4Cwe Location: unknown
• Alliance: Tg(Prnp)C4Cwe page

Transgene origin

• Strain of Origin: B6;129S7-Prnp^tm1Cwe/CweCnrm

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Prnp)C4Cwe expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Prnp (MGI:97769)

• Mutation details: A transgenic construct was engineered to contain murine Prnp lacking sequence encoding amino acids 32 through 93. Sequence, including that encoding the amino-terminal signal sequence, necassary for proper transport to the endoplasmic reticulum and properprocessing was left intact. Transgenic expression was verified by Western blot analysis. Only after treatment with PNGase did the transgenic protein migrate a distance corresponding to the deletion, indicating in vivo glycosylation. A carboxy terminal cleavage product was also detected. The murine Prnp promoter drove expression of similar transgenes (lines 11 and 12) the forebrain and cerebellum. Transgenic transcript was identified in all layers of the cerebellum except for Purkinje cells. Nuclear injections were made in zygotes carrying Prnp^tm1Cwe. (J:47034)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Two founder lines were identified C4Cwe and C15Cwe.

References

• Original: J:47034 Shmerling D, et al., Expression of amino-terminally truncated PrP in the mouse leading to ataxia and specific cerebellar lesions. Cell. 1998 Apr 17;93(2):203-14
• All: 6 reference(s)