Hcst^tm1.1Cln
Targeted Allele Detail

Summary

• Symbol: Hcst^tm1.1Cln
• Name: hematopoietic cell signal transducer; targeted mutation 1.1, Marco Colonna
• MGI ID: MGI:2446491
• Synonyms: DAP10^-
• Gene: Hcst Location: Chr7:30117137-30119279 bp, - strand Genetic Position: Chr7, 17.45 cM
• Alliance: Hcst^tm1.1Cln page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80426
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: This allele is derived from Hcst^tm1Cln by mating mutant animals to cre deleter strain TgN(CMV-cre)1Cgn. Resulting progeny are deleted for the floxed neo cassette and contain a single loxP site in place of exon 1 and intron 1. Absence of gene expression in homozygous mutant animals was confirmed by Western blot analysis of lysates from NK cells and CD8+ cells. (J:80426)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hcst Mutation: 8 strains or lines available

References

• Original: J:80426 Gilfillan S, et al., NKG2D recruits two distinct adapters to trigger NK cell activation and costimulation. Nat Immunol. 2002 Dec;3(12):1150-5
• All: 22 reference(s)