Rdh5^tm1Wsb
Targeted Allele Detail

Summary

• Symbol: Rdh5^tm1Wsb
• Name: retinol dehydrogenase 5; targeted mutation 1, William S Blaner
• MGI ID: MGI:2446073
• Synonyms: cRDH^-
• Gene: Rdh5 Location: Chr10:128749457-128755906 bp, - strand Genetic Position: Chr10, 77.19 cM
• Alliance: Rdh5^tm1Wsb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75693
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The majority of exon 2 and all of exon 3 were replaced by a neomycin selection cassette inserted by homologous recombination. The deleted region encoded approximately two thirds of the wild-type protein. While in situ hybridization of homozygous mutant eye sections using a probe for the targeted region indicated an absence of normal transcript, a truncated transcript was identified by Northern blot analysis. (J:75693)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rdh5 Mutation: 9 strains or lines available

References

• Original: J:75693 Shang E, et al., Targeted disruption of the mouse cis-retinol dehydrogenase gene. Visual and nonvisual functions. J Lipid Res. 2002 Apr;43(4):590-7
• All: 1 reference(s)