Tg(Hba-x-v-Ha-ras)TG.ACLed
Transgene Detail

Summary

• Symbol: Tg(Hba-x-v-Ha-ras)TG.ACLed
• Name: transgene insertion TG.AC, Philip Leder
• MGI ID: MGI:2445993
• Synonyms: TG-AC, TG.AC, Tg(Hba-x-Hras1)1Led, TgN(TG.AC)#Led, Tg(TG.AC)1Led, Tg(TG.AC)#Led
• Transgene: Tg(Hba-x-v-Ha-ras)TG.ACLed Location: unknown
• Alliance: Tg(Hba-x-v-Ha-ras)TG.ACLed page

Transgene origin

• Strain of Origin: FVB/NTac

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The transgene consists of a mouse Hba-x promoter and an Hras1 coding sequence carrying activating mutations G12R (glycine to arginine) and A59T (alanine to threonine). The Hras1 sequence is followed by an SV40 polyadenlyation signal. RNase protection assay detected transgene expression in day 12 embryonic blood, hematopoietic fetal liver, and placenta. Low levels of transgene expression was also detected in adult bone marrow. (J:55016)

Tumor Data

List all tumor models in MMHCdb carrying Tg(Hba-x-v-Ha-ras)TG.ACLed

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Four lines of transgenic mice carrying 3-10 copies of the fusion gene were made. Three of the lines showed no expression of the transgene as measured by RNase protection of an antisense probe. A transgenic founder line designated "TG.AC" was continued.

References

• Original: J:55016 Leder A, et al., v-Ha-ras transgene abrogates the initiation step in mouse skin tumorigenesis: effects of phorbol esters and retinoic acid. Proc Natl Acad Sci U S A. 1990 Dec;87(23):9178-82
• All: 90 reference(s)