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Transgene Detail
Symbol: Tg(GFAP-Hras1)2Agu
Name: transgene insertion 2, Abhijit Guha
MGI ID: MGI:2445442
Synonyms: GFAP-H-RASG12V, GFAP-V12Ha-ras-IRESLacZ, RasB8
Transgene: Tg(GFAP-Hras1)2Agu  Location: unknown  
Alliance: Tg(GFAP-Hras1)2Agu page
Germline Transmission:  Earliest citation of germline transmission: J:69129
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
Tg(GFAP-Hras1)2Agu expresses 1 gene
Mutation detailsThe transgene consists of the human GFAP promoter fused to an Hras1 cDNA, an IRES-lacZ cassette, and a floxed PGK-neo cassette. The human GFAP promoter directs transgene expression in the astrocytes. The Hras1 sequence contains an HA tag at the amino terminus. Western blot analysis of astrocytes from transgenic animals confirmed cell-specific expression of the transgene. Northern blot analysis of brain RNA also confirmed transgene expression. (J:69129)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Tumor Data
List all tumor models in MMHCdb carrying Tg(GFAP-Hras1)2Agu
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
This transgenic is designated RasB8 and upon Southern blot analysis was found to contain a single transgene insertion at a single site.
Original:  J:69129 Ding H, et al., Astrocyte-specific expression of activated p21-ras results in malignant astrocytoma formation in a transgenic mouse model of human gliomas. Cancer Res. 2001 May 1;61(9):3826-36
All:  16 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
MGI 6.22
The Jackson Laboratory