Tg(GFAP-Hras1)2Agu
Transgene Detail

Summary

• Symbol: Tg(GFAP-Hras1)2Agu
• Name: transgene insertion 2, Abhijit Guha
• MGI ID: MGI:2445442
• Synonyms: GFAP-H-RAS^G12V, GFAP-V12Ha-ras-IRESLacZ, RasB8
• Transgene: Tg(GFAP-Hras1)2Agu Location: unknown
• Alliance: Tg(GFAP-Hras1)2Agu page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:69129
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(GFAP-Hras1)2Agu expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Hras (MGI:96224)

• Mutation details: The transgene consists of the human GFAP promoter fused to an Hras1 cDNA, an IRES-lacZ cassette, and a floxed PGK-neo cassette. The human GFAP promoter directs transgene expression in the astrocytes. The Hras1 sequence contains an HA tag at the amino terminus. Western blot analysis of astrocytes from transgenic animals confirmed cell-specific expression of the transgene. Northern blot analysis of brain RNA also confirmed transgene expression. (J:69129)

Disease models

Tumor Data

List all tumor models in MMHCdb carrying Tg(GFAP-Hras1)2Agu

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

This transgenic is designated RasB8 and upon Southern blot analysis was found to contain a single transgene insertion at a single site.

References

• Original: J:69129 Ding H, et al., Astrocyte-specific expression of activated p21-ras results in malignant astrocytoma formation in a transgenic mouse model of human gliomas. Cancer Res. 2001 May 1;61(9):3826-36
• All: 16 reference(s)