Plcb3^tm1Sko
Targeted Allele Detail

Summary

• Symbol: Plcb3^tm1Sko
• Name: phospholipase C, beta 3; targeted mutation 1, Britt Skogseid
• MGI ID: MGI:2445310
• Gene: Plcb3 Location: Chr19:6931081-6951738 bp, - strand Genetic Position: Chr19, 5.1 cM, cytoband B
• Alliance: Plcb3^tm1Sko page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:51860
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 4 kb genomic fragment encompassing exons 11 through 17 was replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region (encoding amino acids 338 through 681) included portions of the X and Y domains, which are putatively essential for enzymatic activity, and a nuclear targeting signal. Western blot analysis showed protein levels to be reduced by approximately 50% in lung tissue obtained from heterozygous mutant mice. (J:51860)

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Plcb3 Mutation: 49 strains or lines available

References

• Original: J:51860 Wang S, et al., Targeted disruption of the mouse phospholipase C beta3 gene results in early embryonic lethality. FEBS Lett. 1998 Dec 18;441(2):261-5
• All: 1 reference(s)