Tcrg^tm1Iku
Targeted Allele Detail

Summary

• Symbol: Tcrg^tm1Iku
• Name: T cell receptor gamma chain; targeted mutation 1, Koichi Ikuta
• MGI ID: MGI:2429947
• Synonyms: TCR-Vgamma4/6 ^-, TCR Vgamma4/6 KO, Vgamma^delta
• Gene: Tcrg Location: Chr13:19362212-19540646 bp, + strand Genetic Position: Chr13, cytoband A2-A3

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80156
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A targeting vector containing a Tcrg-V2 gene modified to lack a restriction site, a floxed Tcrg-V4 gene, and a Tcrg-V3 gene also modified to lack a restriction site was electroporated into ES cells. The ES cells were then transfected with cre recombinase, resulting in the excision of Tcrg-V4. Tcrg-V2 was left adjacent to Tcrg-V3 (with single loxP site in between). Southern blot analysis indicated that only a portion of the targeting vector recombined as the modified Tcrg-V2 and the endogenous Tcrg-V3 (not the modified Tcrg-V3) were present. (J:39791)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcrg Mutation: 1 strain or line available

References

• Original: J:80156 Sunaga S, et al., Efficient removal of loxP-flanked DNA sequences in a gene-targeted locus by transient expression of Cre recombinase in fertilized eggs. Mol Reprod Dev. 1997 Feb;46(2):109-13
• All: 16 reference(s)