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QTL Variant Detail
QTL variant: ModmC57BL/6ByJ
Name: modifier of methylation; C57BL/6ByJ
MGI ID: MGI:2389305
QTL: Modm  Location: unknown  Genetic Position: Chr13, Syntenic
Strain of Specimen:  C57BL/6ByJ
Allele Type:    QTL
Mutation:    Undefined
    This allele confers decreased DNA methylation compared to C57BL/6ByJ. (J:67850)
View phenotypes and curated references for all genotypes (concatenated display).
Animals homozygous for C57BL/6ByJ-derived alleles at Modm exhibited 30%-20% methylation at the transgene locus.

Mapping and Phenotype information for this QTL, its variants and associated markers


QTL mapping was performed on a cross between (BALB/cJ x C57BL/6J)F1 females and E36 transgenic C57BL/6J males. The hepatitis B transgene is differentially methylated depending upon parent of origin. Paternal transmission to a C57BL/6J background results in undermethylation while paternal transmission to a BALB/cJ background results in hypermethylation. Maternal transmission to any genetic background results in methylation. 102 microsatellite markers at an average spacing of 11 cM was typed in 88 (BALB/cJx C57BL/6J)F1 x E36 progeny for QTL mapping. A major locus, Modm (modifier of methylation), was detected on mouse Chromosome 13 spanning 30 cM - 45 cM with a maximum LOD = 16.5 near D13Mit21 and D13Mit97. This locus includes the site of the transgene insertion. Modm was confirmed in 4 CXB (C = BALB/cByJ, B = C57BL/6ByJ) recombinant inbred (RI) strains. CXB RI strains homozygous for BALB/cByJ-derived alleles at Modm exhibited 80%-60% methylation at the transgene locus whereas CXB RI strains homozygous for C57BL/6ByJ-derived alleles exhibit 30%-20% methylation at the transgene locus.

Original:  J:67850 Valenza-Schaerly P, et al., A dominant modifier of transgene methylation is mapped by qtl analysis to mouse chromosome 13. Genome Res. 2001 Mar;11(3):382-8
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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