Cbfb^tm1Ppl
Targeted Allele Detail

Summary

• Symbol: Cbfb^tm1Ppl
• Name: core binding factor beta; targeted mutation 1, Pu P Liu
• MGI ID: MGI:2388355
• Synonyms: Cbfb^Cbfb-MYH11, Cbfb^MYH11, Cbfb-MYH11
• Gene: Cbfb Location: Chr8:105897306-105944621 bp, + strand Genetic Position: Chr8, 53.04 cM
• Alliance: Cbfb^tm1Ppl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:36594
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: MYH11 sequence isolated from a human CBFB-MYH11 cDNA, resulting from a chromosome 16 inversion common in human acute myeloid leukemia subtype M4Eo, was inserted into a StuI site in exon 5 of the endogenous gene by homologous recombination. The targeting vector also contained a neo cassette inserted into intron 5 for selection. Northern and Western blot analyses confirmed the production of fusion transcript and protein in heterozygous mutant embryos. (J:36594)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cbfb Mutation: 36 strains or lines available

References

• Original: J:36594 Castilla LH, et al., Failure of embryonic hematopoiesis and lethal hemorrhages in mouse embryos heterozygous for a knocked-in leukemia gene CBFB-MYH11. Cell. 1996 Nov 15;87(4):687-96
• All: 9 reference(s)