Ciita^tm1Wrth
Targeted Allele Detail

Summary

• Symbol: Ciita^tm1Wrth
• Name: class II transactivator; targeted mutation 1, Walter Reith
• MGI ID: MGI:2388316
• Synonyms: Ciita^IV-, pIV^-
• Gene: Ciita Location: Chr16:10297923-10346282 bp, + strand Genetic Position: Chr16, 5.65 cM, cytoband B1
• Alliance: Ciita^tm1Wrth page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71102
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutation: Intragenic deletion
• Mutation details: The gene was specifically inactivated in non-bone marrow antigen presenting cells by deleting the pIV promoter and its associated exon 4. A loxP site was introduced upstream of pIV and a floxed neo cassette was introduced downstream of exon 4. Transient expression of cre recombinase in correctly targeted ES cells removed the entire floxed region (800 bp) containing the pIV promoter and exon 4. Ablation of type IV mRNA in Ifng-stimulated MEFs (mouse embyronic fibroblasts) derived from homozygous mutant animals was confirmed by RNase protection assay. Gene expression from the pI and pIII promoters was not affected by the pIV deletion. (J:71102)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ciita Mutation: 45 strains or lines available

References

• Original: J:71102 Waldburger JM, et al., Selective abrogation of major histocompatibility complex class II expression on extrahematopoietic cells in mice lacking promoter IV of the class II transactivator gene. J Exp Med. 2001 Aug 20;194(4):393-406
• All: 12 reference(s)