Mcf2^tm1Ehi
Targeted Allele Detail

Summary

• Symbol: Mcf2^tm1Ehi
• Name: mcf.2 transforming sequence; targeted mutation 1, Emilio Hirsch
• MGI ID: MGI:2388053
• Gene: Mcf2 Location: ChrX:59101316-59224449 bp, - strand Genetic Position: ChrX, 33.5 cM, cytoband A6/A7
• Alliance: Mcf2^tm1Ehi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78933
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A cassette containing IRES-lacZ followed by neo was inserted in exon 1 after the start codon via homologous recombination. Transcript was undetected by RT-PCR analysis of testis and brain tissue, while Western blot analysis confirmed the absence of protein in testis, brain, and liver tissue. LacZ expression was undetected by Xgal staining, putatively due to a combination of the low activity of the endogenous promoter and the inefficiency of the IRES sequence. (J:78933)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mcf2 Mutation: 13 strains or lines available

References

• Original: J:78933 Hirsch E, et al., Defective dendrite elongation but normal fertility in mice lacking the Rho-like GTPase activator Dbl. Mol Cell Biol. 2002 May;22(9):3140-8
• All: 1 reference(s)