Htt^tm1.1Tna
Targeted Allele Detail

Summary

• Symbol: Htt^tm1.1Tna
• Name: huntingtin; targeted mutation 1.1, Toshiharu Nagatsu
• MGI ID: MGI:2388035
• Gene: Htt Location: Chr5:34919084-35069878 bp, + strand Genetic Position: Chr5, 17.92 cM
• Alliance: Htt^tm1.1Tna page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76017
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The endogenous exon 1 was replaced with human HD exon 1 sequence containing 77 CAG repeats. The floxed neo cassette was removed from Hdh^tm1Tna by in vivo cre mediated excision in the germline. Western blot analysis of mutant mice showed the protein with the expanded polyglutamine tract to be expressed in a variety of tissues. (J:76017)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Htt Mutation: 179 strains or lines available

References

• Original: J:76017 Ishiguro H, et al., Age-dependent and tissue-specific CAG repeat instability occurs in mouse knock-in for a mutant Huntington's disease gene. J Neurosci Res. 2001 Aug 15;65(4):289-97
• All: 1 reference(s)