Eif2ak4^tm1Drc
Targeted Allele Detail

Summary

• Symbol: Eif2ak4^tm1Drc
• Name: eukaryotic translation initiation factor 2 alpha kinase 4; targeted mutation 1, Douglas R Cavener
• MGI ID: MGI:2387965
• Synonyms: Gcn2^-, Gcn2-Neo
• Gene: Eif2ak4 Location: Chr2:118219099-118305715 bp, + strand Genetic Position: Chr2, 59.4 cM, cytoband E5
• Alliance: Eif2ak4^tm1Drc page

Eif2ak4^tm1Drc/Eif2ak4^tm1Drc mice fed a leucine-devoid diet appear scruffy and lethargic

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78945
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The majority of exon 12, which encodes the kinase subdomains 2 through 4 common to the 3 known isoforms (alpha, beta, and gamma), was replaced by a neomycin selection cassette inserted by homologous recombination. While, RT-PCR analysis showed the presence of an aberrant splice product, sequence analysis showed it generated a frameshift mutation in the open reading frame. Western blot analysis verified the absence of protein. (J:78945)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Eif2ak4 Mutation: 88 strains or lines available

References

• Original: J:78945 Zhang P, et al., The GCN2 eIF2alpha kinase is required for adaptation to amino acid deprivation in mice. Mol Cell Biol. 2002 Oct;22(19):6681-8
• All: 18 reference(s)