Shh^tm3Amc
Targeted Allele Detail

Summary

• Symbol: Shh^tm3Amc
• Name: sonic hedgehog; targeted mutation 3, Andrew P McMahon
• MGI ID: MGI:2387802
• Synonyms: N-Shh, Shhⁿ
• Gene: Shh Location: Chr5:28661838-28672099 bp, - strand Genetic Position: Chr5, 14.39 cM
• Alliance: Shh^tm3Amc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69771
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Single point mutation
• Mutation details: A construct containing a nonsense mutation at cysteine codon 199 was inserted at the endogenous locus via homologous recombination. Termination of translation after glycine 198 putatively precludes autoproteolytic activity and the associated process in which a cholesterol, involved in cell surface tethering, is attached to glycine 198. (J:69771)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Shh Mutation: 45 strains or lines available

References

• Original: J:69771 Lewis PM, et al., Cholesterol modification of sonic hedgehog is required for long-range signaling activity and effective modulation of signaling by Ptc1. Cell. 2001 Jun 1;105(5):599-612
• All: 2 reference(s)